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primary monoclonal antibodies against cd1a  (Novus Biologicals)


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    Novus Biologicals primary monoclonal antibodies against cd1a
    Primary Monoclonal Antibodies Against Cd1a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+monoclonal+antibodies+against+cd1a/CD1a+Antibody+(O10)+-+IHC-Prediluted/pm42033461-57-0-8
    Average 94 stars, based on 1 article reviews
    primary monoclonal antibodies against cd1a - by Bioz Stars, 2026-09
    94/100 stars

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    Bioprocessing:

    Article Title: Localization of dendritic cells and T cells within the tumor microenvironment in different types of skin cancer.
    Article Snippet: For blocking, the tissue sections were incubated in 10% goat serum (Sigma-Aldrich, St. Louis, MO, USA) in 0.5% Triton X-100 (Sigma-Aldrich)/PBS (Gibco) for 1 h at RT in a humidified chamber. .. Primary monoclonal antibodies against CD1a (clone O10, 1:100; Novus Biologicals, Littleton, CO, USA) and CD3 (clone OKT3, 1:100; BioLegend, San Diego, CA, USA) were diluted in 1% bovine serum albumin (BSA, Serva, Heidelberg, Germany)/PBS and applied to each section, followed by incubation overnight at 4 °C in a humidified chamber. .. On day 2, after washing in PBS 3 times for 5 min each, sections were incubated with the appropriate secondary antibodies, anti-mouse IgG2a-Alexa Fluor 594 (1:2000; Invitrogen, Thermo Fisher Scientific) for CD3 and antimouse IgG1-Alexa Fluor 488 (1:1000; Invitrogen) for CD1a, prepared in 1% BSA/PBS.

    Article Title: Localization of dendritic cells and T cells within the tumor microenvironment in different types of skin cancer
    Article Snippet: For blocking, the tissue sections were incubated in 10% goat serum (Sigma-Aldrich, St. Louis, MO, USA) in 0.5% Triton X-100 (Sigma-Aldrich)/PBS (Gibco) for 1 h at RT in a humidified chamber. .. Primary monoclonal antibodies against CD1a (clone O10, 1:100; Novus Biologicals, Littleton, CO, USA) and CD3 (clone OKT3, 1:100; BioLegend, San Diego, CA, USA) were diluted in 1% bovine serum albumin (BSA, Serva, Heidelberg, Germany)/PBS and applied to each section, followed by incubation overnight at 4 °C in a humidified chamber. .. On day 2, after washing in PBS 3 times for 5 min each, sections were incubated with the appropriate secondary antibodies, anti-mouse IgG 2 a-Alexa Fluor 594 (1:2000; Invitrogen, Thermo Fisher Scientific) for CD3 and anti-mouse IgG 1 -Alexa Fluor 488 (1:1000; Invitrogen) for CD1a, prepared in 1% BSA/PBS.

    Incubation:

    Article Title: Localization of dendritic cells and T cells within the tumor microenvironment in different types of skin cancer.
    Article Snippet: For blocking, the tissue sections were incubated in 10% goat serum (Sigma-Aldrich, St. Louis, MO, USA) in 0.5% Triton X-100 (Sigma-Aldrich)/PBS (Gibco) for 1 h at RT in a humidified chamber. .. Primary monoclonal antibodies against CD1a (clone O10, 1:100; Novus Biologicals, Littleton, CO, USA) and CD3 (clone OKT3, 1:100; BioLegend, San Diego, CA, USA) were diluted in 1% bovine serum albumin (BSA, Serva, Heidelberg, Germany)/PBS and applied to each section, followed by incubation overnight at 4 °C in a humidified chamber. .. On day 2, after washing in PBS 3 times for 5 min each, sections were incubated with the appropriate secondary antibodies, anti-mouse IgG2a-Alexa Fluor 594 (1:2000; Invitrogen, Thermo Fisher Scientific) for CD3 and antimouse IgG1-Alexa Fluor 488 (1:1000; Invitrogen) for CD1a, prepared in 1% BSA/PBS.

    Article Title: Localization of dendritic cells and T cells within the tumor microenvironment in different types of skin cancer
    Article Snippet: For blocking, the tissue sections were incubated in 10% goat serum (Sigma-Aldrich, St. Louis, MO, USA) in 0.5% Triton X-100 (Sigma-Aldrich)/PBS (Gibco) for 1 h at RT in a humidified chamber. .. Primary monoclonal antibodies against CD1a (clone O10, 1:100; Novus Biologicals, Littleton, CO, USA) and CD3 (clone OKT3, 1:100; BioLegend, San Diego, CA, USA) were diluted in 1% bovine serum albumin (BSA, Serva, Heidelberg, Germany)/PBS and applied to each section, followed by incubation overnight at 4 °C in a humidified chamber. .. On day 2, after washing in PBS 3 times for 5 min each, sections were incubated with the appropriate secondary antibodies, anti-mouse IgG 2 a-Alexa Fluor 594 (1:2000; Invitrogen, Thermo Fisher Scientific) for CD3 and anti-mouse IgG 1 -Alexa Fluor 488 (1:1000; Invitrogen) for CD1a, prepared in 1% BSA/PBS.



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